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11:16
jove.com
Video: Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
37.2K Views. Promega Corporation. The overall goal of this procedure is to efficiently isolate protein complexes from mammalian cells. This is accomplished by first transecting the cells with a halo tag fusion construct to express the protein of interest. The second step is to slice the cells and covalently capture the protein complexes on ...
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