PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification can be ...

PCR—the workhorse of modern molecular biology—is charging forward using both conventional and digital methods to explore single cells and even single molecules. Nathan Blow reports. Download PDF In ...

A systematic review of 19 cohorts found favorable diagnostic performance for saliva and dried blood spot PCR screening of congenital cytomegalovirus in newborns, with limitations in collection ...

With laboratory methods and diagnostics advancing apace, Francesca Lake considers whether PCR is likely to retain its crown as an essential lab method, or whether it could be replaced and consigned to ...

A diagnostic study demonstrated that a multiplex real-time PCR assay achieved an 81 percent pathogen detection rate in pediatric stool samples compared to 43.1 percent for conventional microbiological ...

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

Robust sterility testing is essential to ensure the safety of cell therapies. However, traditional culture-based analysis takes several weeks to confirm a therapeutic’s microbial contamination, which ...

Wissenschaftler der Physikalisch-Technischen Bundesanstalt (PTB), Institut Berlin, haben die innovative Methode der digitalen Tröpfchen-PCR für den Nachweis von SARS-CoV-2 angepasst und nun in einer ...

Das Kölner Biotechnologie-Start-up Detechgene ist einen entscheidenden Schritt weitergekommen auf dem Weg zum Markteintritt. Die Gründer, der Biologe Reza Esmaillie und der Ingenieur Robin Bayer, ...